Tyrone Possamai
Title: MAPPING OF NEW DISEASES RESISTANCE QTLs-GENES IN GRAPEVINE.
Supervisor: Prof. Raffaele Testolin
Downy and powdery mildews are the most serious diseases affecting grape yield and quality worldwide. Plasmopara viticola and Erysiphe necator are their causal agents.
Resistance trait to mildews has been identified and mapped (QTLs and genes) in different Vitis species. A valid solution to control the pathogens have become the utilize of grapevine varieties with introgressed resistances. Discovering new major resistance QTLs-genes remains an important goal to extend the genetic resources and knowledge to obtain plants with effectives resistances by traditional or precision breeding.
The main objective of this project is the genetic dissection of resistance to E. necator in some less commons Vitis vinifera coming from East-Europe and from Caucasus. For this purpose the strategy is: to cross two sensitive genotypes with two resistant accessions characterized by different levels of tolerance to E. necator; to evaluate the seedlings phenotypic resistance on whole plant and by detached leaf bioassay; and, for a population, to build SNPs based linkage maps for concluding a preliminary QTLs identification.
Subsequently the aims will be to complete the fine mapping of an individuated region and to conclude the resistance putative genes positional cloning. Finally will be also developed targeted markers for the selection (MAS) of new QTLs-genes.
The research activity could be also concerned the isolation of candidate genes for resistance to P. viticola. In this case the work will be completed for an already known QTL.
Biography and Contacts
Tyrone Possamai was born in 1993 in Valdobbiadene (TV) - Italy.
He trained in Viticultural and Oenological Science obtaining the bachelor’s degree at the University of Padua in 2015 and the master’s degree at the University of Turin in 2017, both with honors.
In November 2017 he began the doctoral course at the PhD school of “Agricultural Science and Biotechnology” of the University of Udine and he is carrying out his project in collaboration with the research center CREA-VE in Conegliano (TV).